-40%
New Supelco Discovery HS F5 HPLC Column 15cm x 4.6mm, 5μm # 567516-U
$ 102.96
- Description
- Size Guide
Description
Winner will receive one (1) Supelco Discovery HS F5 HPLC Column 15cm x 4.6mm, 5μm - Part# 567516-UItem is old unused stock from a closed laboratory. It is sealed in original box with instruction sheet and certificate of analysis.
Features and Benefits
• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes
General description
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P
o/w
values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
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